Single Vesicle
Proximity Barcoding Assay (svPBA)
Single-EV, high-throughput profiling with optimized antibody panels and tailored PBA templates — revealing vesicle heterogeneity and accelerating biomarker and drug target discovery.
Working steps of svPBA
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1
EV Capture — immobilization of EVs via affinity capture.
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2
Probe Binding — PBA probe panel targets EV surface proteins.
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3
Hybridization — DNA templates hybridize with ssDNA labels of PBA probes.
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4
Proximity Barcoding — Unique EV tags added on the ssDNA labels of each single EVs.
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5
Amplification — PCR enriches the sequences containing EV–protein–molecule tags.
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6
Library Prep — Sample indexing and sequencing library construction.
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7
Decoding — NGS and bioinformatics reconstruct single-EV molecular profiles.
Vesicode®Surface 550
- 550+ validated antibodies for EV surface proteins.
- Designed for biomarker discovery and drug target screening.
- Functional coverage of EV biology and targeting.
- Includes immune cell-type markers.
Vesicode®Mouse 200
- 200+ validated antibodies tailored for mouse EV surface proteins.
- Optimized for preclinical studies in murine models.
- Coverage includes key immune markers.
- Enabling studies on EV biology and functions.
svPBA Kits
- End-to-end experimental kits designed for standard laboratory workflows.
- All-in-one solution for EV capture, probe labeling, proximity barcoding, and library preparation.
- Ready-to-use protocols ensuring reproducibility and scalability across experiments.
- Flexible formats to fit diverse research projects and study scales.
| Quantity | Cat.nr. |
|---|---|
| 8 | KEV001M8 |
| 16 | KEV001M16 |
| 24 | KEV001M24 |
| 48 | KEV001M48 |
| 96 | KEV001M96 |
